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Streptococcus Agalactiae Discussed in PhD Thesis

Published Date : 13/12/2018
No. of Viewers : 597
Edited By : University of Babylon

A PhD thesis was discussed at the Faculty of Medicine (description of Streptococcus agalactiae of pregnant women), for the student Tayseer Safa Jawad, under the supervision of Dr. Lamis Abdel Razzaq, Dr. Asma Kazem Kobey, a number of faculty and postgraduate students being present.

The researcher used (300) vaginal sample obtained from women suffering from vaginal infections, and the samples were planted in selective circles and identified using biochemical and bacteriological tests and the use of the specialist initiator (ATR).

Only 25 isolates of Streptococcus agalactiae were collected and the use of the polymerase chain Reaction technique (PCR) for the investigation of Genes pathogenicity SCPB, cyle using a specific starter on gene DNA from the isolation of  Streptococcus agalactiae indicated the amplification of PCR by all the isolates having Jane SCPB, Cyle.

The researcher was able to detect the genes of Pi-1, PI-2A, Pi-2b by the PCR technique using a specific initiator, and found that the genes exist in ratios (76, 24, 60%) respectively. The DNA sequence of gene Cyle from the isolates of Streptococcus agalactiae bacteria revealed 14 mutations in four isolates while one isolation was 100% identical and gave a variation of (94,100%) with Sstreptrep. Agalactiae is a standard site in NCBI and is the first registration in Iraq.